| Concentration (µg∙mL−1) | Fractions | |||
| EAF | VLC2 | VLC3 | VLC4 | |
| 5000 | + | + | + | + |
| 2500 | + | + | + | + |
| 1250 | + | + | + | − |
| 625 | + | + | + | − |
| 312.5 | + | + | + | − |
| 156.25 | − | + | + | − |
| 78.12 | − | − | − | − |
| 39.06 | − | − | − | − |
| 19.53 | − | − | − | − |
| 9.76 | − | − | − | − |