Method

Advantage

Disadvantage

Mainly References

Dose

There are conversion formulas such as weight or body surface area; More accurate calculation

The effective amount used in different animals is inconsistent; Excessive dose can cause death of small animals

[13] [15]

Speed

Fast onset, high bioavailability, easy to control blood concentration

The speed is difficult to control, resulting in inaccurate results; Need infusion pump, syringe pump and other instruments to control the speed

[15] [21] [22] [27]

Concentration

Simple and convenient to use the dilution method

There is no standard conversion formula to rely on; Inaccurate estimates can lead to animal poisoning reactions

[15] [21] [22]

Time

Effectiveness, increase the success rate of modeling;

Correlation, associated with dose, speed, and concentration

There is a lack of standard standards for medication time, time interval and days of modeling

[18] [25] [27]