Method

Vapor diffusion

Plate type

Hanging-drop 24 wells

Temperature (K)

293 K

Protein concentration

10 mg/ml

Buffer composition of protein solution

50 mM Tris-HCl (pH 8.0)/0.2 M NaCl/4 mM DTT

Composition of reservoir solution

13% (w/v) PEG6000, 0.1 M sodium cacodylate buffer at pH 6.0, 0.1 M MgCl2, 2 mM sodium dithionite, 10 mM dithiothreitol

Volume and ratio of drop

4 μl 1:1

Volume of reservoir

1 ml