Method | Vapor diffusion |
Plate type | Hanging-drop 24 wells |
Temperature (K) | 293 K |
Protein concentration | 10 mg/ml |
Buffer composition of protein solution | 50 mM Tris-HCl (pH 8.0)/0.2 M NaCl/4 mM DTT |
Composition of reservoir solution | 13% (w/v) PEG6000, 0.1 M sodium cacodylate buffer at pH 6.0, 0.1 M MgCl2, 2 mM sodium dithionite, 10 mM dithiothreitol |
Volume and ratio of drop | 4 μl 1:1 |
Volume of reservoir | 1 ml |