MRM Precursor

Ion

(m/z)

MRM

Product Ion (m/z)

DP

(V)

CE

(V)

CXP

(V)

Retention Time (min)

Target Analyte

17β-estradiol

255^*

159

66

21

14

22.2

Estrone

253^*

197

66

29

18

20.4

Estriol

271^*

133

51

35

10

14.5

Zearalanol

305^*

189

61

27

12

18.5

Zearalenone

301^*

283

71

23

24

23.5

Zearalanone

321

303

36

19

18

22.8

Testosterone

289

109

81

33

6

23.9

5α-androstan-17b-ol-3-one

273^*

255

66

21

6

26.5

Androsterone

273^*

255

51

17

16

27.1

5α-androstane-3,17-dione

289

272

41

17

18

23.8

4-androstene-3,17-dione

287

97

41

33

6

21.6

Boldenone

287

121

11

33

8

20.9

Nandrolone

275

109

56

39

8

22.1

17β-trenbolone

271

253

71

31

22

20.9

17α-trenbolone

271

253

56

29

16

20.6

Progesterone

315

109

61

35

8

27.1

17,20-dihydroxyprogesterone

333

271

56

27

18

23.9

Melengestrol

355

337

46

25

20

24.5

Melengestrol acetate

397

337

36

21

20

26.9

Isotopically-labeled Analogue

17β-estradiol d5

260^*

162

51

25

10

22.1

Estriol-d3

274^*

256

61

17

16

14.5

α-zearalenol-d4

325

288

16

17

18

19.5

Testosterone-d5

294

100

71

33

6

23.7

Testosterone d3

292

97

56

33

16

23.7

17β-trenbolone-d3

274

256

66

29

16

20.8

Progesterone d9

325

100

121

39

8

27.0

Melengestrol-d3

358

282

56

29

18

26.8

Melengestrol acetate-d3

400

340

46

21

20

24.4