| Extraction/ cleanup | Instrument | Matrix | Hormones targeted1 | Spike conc. (ng・g−1) | Efficiency (%) | RSD (%) | LOD (ng・g−1) | Internal surrogates | Ref. |
| ultrasonic/SPE (C18) | LC-DAD-MS2 LC-ESI-MS3 | 5 g lyophilized sediment | E1, E2, E3, P | 1000 | DAD 66 - 102 ESI 64 - 100 | DAD 9 - 11 ESI 6 - 19 | 0.04 - 1.00 | none | [10] |
| ASE/SPE (C18) | LC-ESI-MS3 | 5 g lyophilized sediment | E1, E2, E3 | 500 | 78 - 94 | 9 - 13 | 2.00 - 10.0 | none | [11] |
| sonication, Soxhlet/silica | PFPA4 derivatization and GC-MS5 | 10 g pre-extracted (Soxhlet) sediment | E1, E2, E3 | 100 | sonication 32 - 132 Soxhlet 37 - 133 | 3 - 5 3 - 14 | 0.60 - 2.50 | 17β-estradiol acetate | [12] |
| MASE6/SPE (Strata-X-AW) and silica | LC-ESI-MS3 | 1 g wet sediment | E1, E2, EE2 | 10 | 82 - 98 | 10 - 16 | 4.00 - 8.00 | E1-d4 E2-d4, EE2-d4 | [13] |
| Soxhlet/ alumina-florisil | BSTFA:TMCS7 derivatization and GC-MS5 | 10 g dried solid | E1, E2, 17βE2, 4A, AND, P, T | 10, 50 | 95 - 150 | 5 - 25 | 0.02 - 0.07 | 17βE2-d4, P-d9 | [14] |
| ASE/SPE (C18) | MSTFA8 derivatization and GC-MS5 | 30 g dried and sieved soil | E1, 17βE2, E3 | 2 | 89 - 103 | 10 - 13 | 0.05 | 17βEE2-d2 | [15] |
| ASE/RAM9 | LC-MSD-API-ESI10 | 5 g lyophilized and sieved sediment | E1, E2, E3, P | 100 | 94 - 104 | 2 - 7 | 0.50 - 5.00 | none | [16] |
| ultrasonic/SPE (HLB) | GC-MS5 (no derivatization) | 3 g dried sediment | E1, E2, E3 | 15, 150 | 60 - 127 58 - 133 | 5 - 17 4 - 12 | 1.50 - 5.00 | 13C2-EE2, 17βE2-d3 | [17] |