Plant growth regulators (mg/l)

Concentration

of

AgNO3 (mg/l)

Regeneration

frequency

(%)

Mean no. of shoots/explant

Mean shoot length (cm)

Callus formation

BAP

Kn

NAA

IAA

1.0

-

0.5

-

-

70

9.5 ± 0.36

3.4 ± 0.29

C+

1.0

-

0.5

-

0.4

75

18.4 ± 0.21

6.5 ± 0.1

-

2.0

-

0.5

-

-

85

10.4 ± 0.10

7.4 ± 0.26

-

2.0

-

0.5

-

0.4

90

34.3 ± 0.25

10.3 ± 0.31

-

3.0

-

0.5

-

-

70

6.5 ± 0.36

4.7 ± 0.05

C++

3.0

-

0.5

-

0.4

75

17.3 ± 0.10

4.3 ± 0.15

-

1.0

-

-

0.5

-

65

7.9 ± 0.61

3.4 ± 0.2

-

1.0

-

-

0.5

0.4

70

15.5 ± 0.32

5.8 ± 0.25

-

2.0

-

-

0.5

-

75

12.2 ± 0.15

6.5 ± 0.18

-

2.0

-

-

0.5

0.4

80

22.4 ± 0.23

11.3 ± 0.37

-

3.0

-

-

0.5

-

70

9.8 ± 0.55

2.4 ± 0.18

-

3.0

-

-

0.5

0.4

65

13.5 ± 0.15

8.7 ± 0.26

-

-

1.0

0.5

-

-

60

6.9 ± 0.73

4.3 ± 0.37

C+

-

1.0

0.5

-

0.4

70

14.7± 0.45

6.5 ± 0.20

-

-

2.0

0.5

-

-

65

11.4 ± 0.22

5.5 ± 0.35

C+

-

2.0

0.5

-

0.4

82

16.4 ± 0.32

8.5 ± 0.2

-

-

3.0

0.5

-

-

60

5.5 ± 0.20

3.8 ± 0.36

-

-

3.0

0.5

-

0.4

70

12.7 ± 0.4

5.2 ± 0.20

-

-

1.0

-

0.5

-

55

5.7 ± 0.14

3.8 ± 0.25

-

-

1.0

-

0.5

0.4

65

10.5 ± 0.34

5.4 ± 0.30

-

-

2.0

-

0.5

-

65

8.98 ± 0.42

6.4 ± 0.18

-

-

2.0

-

0.5

0.4

70

14.2 ± 0.15

7.5 ± 0.34

-

-

3.0

-

0.5

-

60

4.5 ± 0.16

4.2 ± 0.14

-

-

3.0

-

0.5

0.4

65

12.4 ± 0.2

4.9 ± 0.26

-