| Primer name | Primer sequence (5’-3’) | Restriction site | Gene size on ORF | Expected product size |
| PCR amplification primers | ||||
| NS2-F | GC GAT ATC CTG GAC ACG GAG GTG GCC | EcoRV | 651 bp | 651 bp |
| NS2-R | AAT CTA GA TTA CAG CAA CCT CCA CCC CTT | Xba I | ||
| NS4A-F | GC GAT ATC AGC ACC TGG GTG CTC GTT | EcoRV | 162 bp | 165 bp |
| NS4A-R | AAT CTA GA TTA GCA CTC TTC CAT CTC ATC | Xba I | ||
| NS5A-F | GC GAT ATC TCC GGT TCC TGG CCA AGG | EcoRV | 1344 bp | 1344 bp |
| NS5A-R | AAT CTA GA TTA GCA GCA CAC GAC ATC TTC | Xba I | ||
|
| EcoRV 5’-GC GAT ATC-3’ forward Xba I 5’-AAT CTA GA TTA-3’ reverse |
|
|
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| FlagTAG Primers | ||||
| FlagTAG-sense BamHI-5’-GATCC ATG GAC TAC AAG GAC GAC GAT GAC AAG GAC TAC AAG GAC GAC GAT GAC AAG GAT-3’-EcoRV | ||||
| FlagTAG-antisense EcoRV-5’-ATC CTT GTC ATC GTC GTC CTT GTA GTC GTT GTC ATC GTC GTC CTG GTA GTC CAT G-BamHI | ||||
| RT-PCR primers | ||||
| NS2-F | GGACGACGATGACAAGGACT |
|
| 383 bp |
| NS2-R | CGCACGAAGTAGGGGACTT |
|
| |
| NS4A-F | AGCACCTGGGTGCTCGTT |
|
| 140 bp |
| NS4A-R | AGGTATAATTGCCGGCTTCC |
|
| |
| NS5A-F | GGACGACGATGACAAGGACT |
|
| 260 bp |
| NS5A-R | TTATAGTTCGGCGCAGGAAG |
|
| |
| GAPDH-F | ACCACAGTCCATGCCATCAC |
|
| 453 bp |
| GAPDH-R | TCCACCACCCTGTTGCTGTA |
|
| |