| Gene | Description | PCR products (bp) | Primer sequence (5′to 3′)a | |
| pTJ100-related genes | ||||
| iucA | Aerobactin production | 236 | F-CGCGAGCGGCTCATACAGG | |
| R-TCGTCGGGCAGCGTTTCT | ||||
| iucD | The aerogenes gene | 400 | F-AGTTCTATCGCTTCCTTAC | |
| R- GAGACCCAGTTTATTTCC | ||||
| iutA | Ferric aerobactin receptor gene | 424 | F-AACAAACCGATGATGAAACG | |
| R-GTGCCAGCCTCAAACTCC | ||||
| iss | Increased serum survival gene | 756 | F-GTTCTCCGTCGGGCTACT | |
| R-GCTCTGCGTGATGATGTT | ||||
| tsh | Temperature-sensitive hemagglutinin gene | 488 | F-ACGGTCAATAATGAACTCG | |
| R-CAGGAATATGCACCTCCC | ||||
| Iron-related genes | ||||
| irp2 | Iron repressible gene | 236 | F-CGCGAGCGGCTCATACAGG | |
| R-TCGTCGGGCAGCGTTTCT | ||||
| fyuA | Gene of the pesticin receptor of Yersinia | 235 | F-ACCGTTATCGCCATTCTG | |
| R-CTGTGAAGTCTGGGCATTAG | ||||
| Adhesins-related genes | ||||
| papA | Genes encoding parts of the P pilus | 374 | F-GCTCCAACTATTCCACAG | |
| R-TTCAGGGTATTAGCATCAC | ||||
| papC | Genes encoding parts of the P pilus | 234 | F-GGGCGTGATAACGATTC | |
| R-ATTTGCCAGCGGACTAC | ||||
| eaeA | Encoding outer membrane protein intimin | 827 | F-GCGTTACATTGACTCCC | |
| R-CATTGCTACCACCTTGC | ||||
| FimA | Genes encoding parts of the F1 pilus | 352 | F-CAGGTTCGTACCGCATCG | |
| R-TCGCATCCGCATTAGCAG | ||||
| FimC | The Type 1 fimbrial adhesin | 337 | F-GCCGATGGTGTAAAGGAT | |
| R-CCGTCAGGTAATAGGGTGT | ||||
| Toxins-related genes | ||||
| vat | Toxin gene | 861 | F-TAAATGAGGTGGGCTGTG | |
| R-AGGATGCCTCCGTAAACT | ||||
| stx1 | Shiga toxin 1 | 374 | F-GCCATTCGTTGACTACTT | |
| R-CTCATCAGATGCCATTCT | ||||
| stx2 | Shiga toxin 2 | 240 | F-ACTGTCTGAAACTGCTCC | |
| R-TGACATTCTGGTTGACTCT | ||||
| hlyA | Transport gene of the hemolysin operon | 290 | F-TTGGGATACGCTGATAGG | |
| R-CACCCTTGACTAATAACTCG | ||||
| Other factors | ||||
| colV | ColV production | 307 | F-ACGGATGCTCAGTTTCT | |
| R-TGTGCTTGGCGTCATAG | ||||
| colBM | Colicins B and colicins M | 226 | F-GAGCCTGCTGTCACCCTT | |
| R-GTTCAGATAATGCCCGATG | ||||