Run | X1 Molasses (g/L) | X2 Nitrate (g/L) | X3 Inoculum size (ml) | X4 Medium volume (ml) | Response (Y) E24 |
1 | 15(0) | 15(0) | 2(0) | 60(2) | 79.4 |
2 | 10(−1) | 20(1) | 1.5(−1) | 50(1) | 58 |
3 | 15(0) | 15(0) | 2(0) | 40(0) | 73.2 |
4 | 10(−1) | 20(1) | 1.5(−1) | 30(−1) | 81.8 |
5 | 20(1) | 10(−1) | 2.5(1) | 50(1) | 85.6 |
6 | 20(1) | 20(1) | 1.5(−1) | 30(−1) | 69.3 |
7 | 15(0) | 5(−2) | 2(0) | 40(0) | 80 |
8 | 10(−1) | 20(1) | 1.5(−1) | 30(−1) | 73 |
9 | 15(0) | 15(0) | 2(0) | 40(0) | 86.7 |
10 | 20(1) | 10(−1) | 1.5(−1) | 50(1) | 88.3 |
11 | 15(0) | 15(0) | 2(0) | 40(0) | 77 |
12 | 15(0) | 25(2) | 2(0) | 40(0) | 78.8 |
13 | 15(0) | 15(0) | 2(0) | 40(0) | 71 |
14 | 15(0) | 15(0) | 1(−2) | 40(0) | 51 |
15 | 10(−1) | 10(−1) | 2.5(1) | 50(1) | 55.3 |
16 | 20(1) | 20(1) | 2.5(1) | 50(1) | 78.4 |
17 | 15(0) | 15(0) | 2(0) | 40(0) | 73.2 |
18 | 10(−1) | 10(−1) | 2.5(1) | 30(−1) | 55 |
19 | 10(−1) | 10(−1) | 2.5(1) | 50(1) | 70 |
20 | 25(2) | 15(0) | 2(0) | 40(0) | 93.8 |
21 | 10(−1) | 20(1) | 2.5(1) | 50(1) | 52.8 |
22 | 15(0) | 15(0) | 2(0) | 40(0) | 72 |
23 | 20(1) | 20(1) | 2.5(1) | 30(−1) | 60 |
24 | 20(1) | 10(−1) | 2.5(1) | 30(−1) | 60 |
25 | 15(0) | 15(0) | 3(2) | 40(0) | 60 |
26 | 10(−1) | 10(−1) | 1.5(−1) | 30(−1) | 53 |
27 | 20(1) | 20(1) | 1.5(−1) | 50(1) | 95 |
28 | 15(0) | 15(0) | 2(0) | 40(0) | 71 |
29 | 5(−2) | 15(0) | 2(0) | 40(0) | 55 |
30 | 15(0) | 15(0) | 2(0) | 20(−2) | 65.2 |
31 | 20(1) | 10(−1) | 1.5(−1) | 20(−2) | 59.3 |