| Protein | Primer type | Nucleotide sequence | Length (bp) |
| CpMDH | Forward | 5’-CATATGAAAGTAGCTGTTTTAGG-3’ | 23 |
| Reverse | 5’-CTCGAGGCTAGCCATAAAATCAA-3’ | 23 | |
| CpCSY | Forward | 5’-CATATGGCTGAATCAAAAGCCA-3’ | 23 |
| Reverse | 5’-CTCGAGTCTTTTATTTAATGGTA-3’ | 23 | |
| EcMDH | Forward | 5’-CATATGAAAGTCGCAGTCCTC-3’ | 21 |
| Reverse | 5’-CTCGAGCTTATTAACGAACTCTT-3’ | 23 | |
| EcCSY | Forward | 5’-CATATGGCTGATACAAAAGCAA-3’ | 22 |
| Reverse | 5’-CTCGAGACGCTTGATATCGCTTT-3’ | 23 |