(a) NH4+

(b) NO2

(c) NO3

0 h

1 h

3 h

6 h

9 h

24 h

48 h

96 h

0 h

1 h

3 h

6 h

9 h

24 h

48 h

96 h

0 h

1 h

3 h

6 h

9 h

24 h

48 h

96 h

kDa

NH4+

0

0

0

0

0

0

0

0

38.1

39.9

19.1

10.1

10.0

39.9*

29.6*

27.5#

100

44.5

19.8

35.8

0

0

0

0

58

0

0

0

100*

100*

100*

100*

100*

61.9

60.1

80.9

89.9

90.0

60.1*

70.4*

72.5#

0

55.5

80.1

64.2

100*

100*

100*

100*

44

NO2

0

0

0

39.3

35.0

17.4

22.9

41.4

41.0

37.3

63.8

63.3

52.0

61.2

67.1

59.1

86.9

63.2

38.9

37.6

20.5

28.8

58.6

54.3

58

100*

100*

100*

60.6

64.9

82.6

77.0

58.5

58.9

62.7

36.2

36.7

47.9

39.0

32.8

40.8

13.1

36.8

61.1

62.3

79.4

71.2

41.4

45.7

44

NO3

0

0

73.4

80.9

79.5

77.2

76.4

63.3

50.7

50.5

83.4

81.6

83.4

78.5

72.4

63.3

63.2

63.1

83.8

90.0

85.9

83.4

79.9

62.4

58

100*

100*

26.6

19.1

20.5

22.8

23.6

36.7

49.3

49.5

16.6

18.4

16.5

21.4

27.6

36.7

36.8

36.9

16.2

9.9

14.0

16.6

20.0

37.6

44

N-free

0

0

100

100

100

100

100

100

40.1

49.0

84.7

66.4

64.1

81.9

77.4

82.8

81.8

100

100

100

100

100

100

71.1

58

0

0

0

0

0

0

0

0

59.9

50.9

15.3

33.6

35.9

18.1

22.6

17.2

18.2

0

0

0

0

0

0

28.9

44