| RecA Primer CCGTTTATGCGGAACACCTA TCGGGATTACCAAACATCAC Torriani et al. (2001) | Volume of components | Orf-hisG primer CTTCGTTATGATTTTACA CAATATCAACAATTCCAT Coroller et al. (1999) | Volume of components |
10× |
| 25 µl |
| 25 µl |
dNTP mix 10 mM |
| 5 µl |
| 5 µl |
Forward primer |
| 10 µl |
| 15 µl |
Reverse primer |
| 10 µl |
| 15 µl |
25 mM Mgcl2 |
| 15 µl |
| 30 µl |
Template DNA |
| 2 µl |
| 3 µl |
Taq polymerase 5 U/µl |
| 1.5 µl |
| 2 µl |
Water, nuclease free |
| 175 µl |
| 146 µl |
Total volume |
| 250 µl |
| 250 µl |