| Stage | Step | Temperature (˚C) | Time |
| Hold | Initial denaturation | 95 | 10 min |
| 43 cycles | Denature | 95 | 30 sec |
| Annealing/Extension | 60 | 1.5 min | |
| Hold | Final Extension | 72 | 7 min |
| Hold | Final hold | 4 | ∞ |