Parameter

Optimum value

Error

Efficiency

Slope

R²

Annealing temperature for primers

52˚C

0.0094

1.963

−3.415

0.9914

glmMRv primer concentration

1 µM

0.0374

1.962

−3.416

0.9944

glmMFw primer concentration

1 µM

0.0374

1.962

−3.416

0.9944

Probe concentration

1 µM

0.0094

1.963

−3.415

0.9914