| Time, min | K2HPO4%: 100 mM Tris HCl + 25% Acetontrile | TEAB %: 100 mM Tris HCl + 25% Acetonitrile + 1.25 M NaCl |
| 0 | 100 | 0 |
| 30 | 50 | 50 |
| 31 | 50 | 50 |
| 33 | 100 | 0 |
| 36 | 100 | 0 |