| Preparative Chromatographic Conditions | |
| Column | X-Select HSS CN OBDTM (250 × 19 5 µ) |
| Mobile phase | Acetonitrile (B), 10 mM Ammonium bicarbonate (A) |
| Programme | Gradient |
| Flow rate | 20 mL/min |
| Wavelength | 280 |
| Column temperature | Ambient |
| Injection volume | 500 µL |
| Diluent | Mobilephase |
| Gradient programme | (Time/%B): 0/5, 4/5, 10/50, 10.1/98, 12/98, 12.1/5, 14/5 |
| Source voltages | |
| Capillary (kV) | 3.5 |
| Cone (V) | 35 |
| Source Temperatures | |
| Disolvation Temperature (˚C) | 350 |
| Source Gas flow | |
| Desolvation Gas flow (L/Hr) | 650 |
| Cone (L/Hr) | 50 |
| Analyser | |
| LM Resolution | 15 |
| HM Resolution | 15 |
| Ion Energy | 0.5 |
| Mass range | 150 - 850 |