Analytical chromatographic conditions | |
Column | Eclipse Plus C18 (50 × 4.6 1.8 μ) |
Mobile phase | Acetonitrile (B), 10mM Ammonium bicarbonate (A) |
Program | Gradient |
Flow rate | 0.6 mL/min |
Wavelength | 280 nm |
Column Temperature | 35˚C |
Injection volume | 2 μL |
Diluent | Mobile phase (50:50) |
Gradient program | 0/3, 0.3/3, 3.2/98, 4/98, 4.1/3 |
(Time/%B) |