| Mobile phase | Mobile phase | ||||||
| Water: ACN (50:50) | MeOH: Formic acid (50:50) | ||||||
| Ammonium acetate: ACN (50:50) | MeOH: Ammonium hydroxide (50:50) | ||||||
| ACN: MeOH (50:50) | ACN: Formic acid (50:50) | ||||||
| ACN | ACN: Ammonium hydroxide (50:50) | ||||||
| Compound | RTa (min) | Precursor Ion (m∙z−1) | Product Ion (m∙z−1) | DPc (V) | EPd (V) | CCe (V) | CCEPf (V) |
| 17 β-estradiol | 1.06 | 271.0 | 239.2, 145.3, 144.4, 143.1b | −150 | −14 | −70 | −10 |
| Estrone | 1.41 | 269.2 | 158.9, 145.1b, 142.5 | −150 | −7 | −40 | −15 |
| 17 α-ethinylestradiol | 1.27 | 295.1 | 213.2, 198.9, 170.6, 159.0b, 144.7, 142.4 | −160 | −7.7 | −60 | −20 |
| Estriol | 0.56 | 287.1 | 255.0, 210.8, 171.1b, 158.8, 144.7 | −150 | −9 | −60 | −15 |