| N-gene | ||||||||
| Concentration | Ct1 | Ct2 | Ct3 | Ct4 | Ct5 | Mean | SD | %CV |
| S1 | 17.951 | 17.785 | 17.957 | 18.062 | 18.037 | 17.9584 | 0.108438 | 0.603829 |
| S2 | - | - | - | - | - | - | - | - |
| S3 | 28.577 | 28.893 | 28.862 | 28.805 | 28.996 | 28.8266 | 0.155825 | 0.540558 |
| S4 | 22.992 | 23.147 | 23.078 | 23.265 | 23.102 | 23.1168 | 0.100218 | 0.43353 |
| ORF1ab | ||||||||
| Concentration | Ct1 | Ct2 | Ct3 | Ct4 | Ct5 | Mean | SD | %CV |
| S1 | 19.175 | 19.093 | 19.265 | 19 | 19.095 | 19.1652 | 0.072926 | 0.380513 |
| S2 | 36.806 | 37.774 | 37.263 | 38.299 | - | 37.5355 | 0.644526 | 1.71711 |
| S3 | 31.697 | 31.917 | 31.657 | 31.833 | 31.882 | 31.7972 | 0.114587 | 0.360368 |
| S4 | 23.927 | 24.147 | 24.029 | 24.283 | 24.085 | 24.0942 | 0.132971 | 0.551878 |