| Primary antibodies | Detection system | Type | Dilution | Reference |
| Anti Estrogen Receptor | Avidin-biotin immunoperoxidase | Mouse Monoclonal | 1:50 | Manee-in et al. [29] |
| Anti Progesterone Receptor | Avidin-biotin immunoperoxidase | Mouse Monoclonal | 1:100 | Manee-in et al. [29] |
| Anti Androgen Receptor | Streptavidin-biotin-complex peroxidase | Rabbit Policlonal | 1:15 | Illera et al. [33] |
| Anti p53 | Streptavidin-biotin-complex Peroxidase | Mouse Monoclonal | 1:50 | Kanavaros et al. [27] |
| Anti-HER-2 | horseradish peroxidase-conjugated | Rabbit Policlonal* | 1:100 | Bertagnolli et al. [34] |
| Anti Ki-67 | Alkalinephosphatase/anti-alkaline phospatase | Mouse Monoclonal | 1:20 | Kanavaros et al. [27] |
| Anti-CD31 | Avidin-biotin immunoperoxidase | Mouse Monoclonal | 1:100 | Kanavaros et al. [27] |
| Anti-p16, p21, p27 | Horseradish peroxidase-conjugated | Mouse Monoclonal | 1:50; 1:100; 1:50 | MacNeil et al. [35] Kanavaros et al. [27] |