| Primer name | Sequence (5'-3') | Length | Usage | |
| Oligo dT | CTGTGAATGCTGCGACTACGATTTTTTTTTTTTTTTTTTT | 40mer | cDNA synthesis | |
| Chi-a (F) | TGYTAYTTYACNAAYTGG | 19mer | Conserved region PCR | |
| Chi-b (F) | GAYATHGAYTGGGARTAYCC | 19mer | ||
| Chi-c (R) | TTCCARTARTTCATNGCRTARTC | 19mer | ||
| 3’RACE (R) | CTGTGAATGCGACTACGAT | 19mer | 3'RACE PCR | |
| AAP (F) | GGCCACGCGTCGACTAGTACGGGIIGGGIIGGGIIG | 36mer | 5'RACE PCR | |
| AUAP (F) | GGCCACGCGTCGACTAGTACC | 21mer | ||
| β-actin 1 (F) | AGCCAACAGAGGAGGCTCTTA | 21mer | Tissue expression PCR | |
| β-actin 2 (R) | GATTCCTAAGAGTGAATG | 18mer | ||
| Chub mackerel | SjChi1-1(F) | ACCAACATTGACCCATGTCTCTGTG | 25mer | 3'RACE PCR |
| SjChi1-2(F) | ATGGACAACAACATGATCAAGAC | 23mer | ||
| SjChi2-1(F) | GGTGAGTGCAGTCCCCTGTTCA | 22mer | ||
| SjChi1-3(R) | CTCCAATGGCCAACAGAGTCTTCA | 24mer | 5'RACE PCR | |
| SjChi1-4(R) | TGGAACTGTCCGTAGAGTTTTTC | 23mer | ||
| SjChi1-5(R) | GTTGTTGTCCATTGATGCAAAGG | 23mer | ||
| SjChi1-6(R) | GGTCAATGTTGGTGGGTAGGTACT | 24mer | ||
| SjChi2-2(R) | AAGACGAATAGTCTAAGGGTT | 21mer | ||
| SjChi2-3(R) | GAATCAATGGTGTCCTTTCCA | 21mer | ||
| SjChi2-4(R) | ACAGCAGCAGACATCAGAAGACG | 24mer | ||
| SjChi1-7(F) | TACAGAAGCAATATGGGCAAACTACTC | 27mer | Full length amplification | |
| SjChi1-8(R) | AGTGGAATGGTCTTGTGTTTCATGATA | 27mer | ||
| SjChi2-5(F) | CGGTAGCCATGGGGAAAGTACTG | 23mer | ||
| SjChi2-6(R) | ACAACAGTTATCCAGTCAATTTAT | 24mer | ||
| SjChi1-9(F) | CATCCACGTCATGTCCTACGA | 21mer | Tissue expression PCR | |
| SjChi1-10(R) | CGTTGTTGTAGGCATATGGCA | 21mer | ||
| SjChi2-8(F) | GTCATATGACTTCCATGGCTC | 21mer | ||
| SjChi2-9(R) | CCCACTGATTTCCCTTGTAAG | 21mer | ||
| Silver croaker | PaChi1-1(F) | GGACTACTTCCACGTCATG | 19mer | 3’RACE PCR |
| PaChi2-1(F) | TGTGGACTACGCCATGAACTAC | 19mer | ||
| PaChi1-2(R) | CCACATTGAAGTAGATCAT | 19mer | 5’RACE PCR | |
| PaChi1-3(R) | CATGACGTGGAAGTAGTCC | 19mer | ||
| PaChi1-4(R) | GCGAGGACGGTTGGTCTTCT | 20mer | ||
| PaChi2-3(R) | GTAGTTCATGGCGTAGTCCACA | 22mer | ||
| PaChi2-4(R) | GAAATAGATGAAGCCACCC | 19mer | ||
| PaChi2-5(R) | GGCCGAGCTTGGGGATCTGAT | 21mer | ||
| PaChi1-5(F) | TACAGAAGCACCATGGGCAAGCTAC | 25mer | Full length amplification | |
| PaChi1-6(R) | GCTTTAAATGCGACGTTTATTTAA | 24mer | ||
| PaChi2-6(F) | TACACGGTAGCCATGGGGAAA | 21mer | ||
| PaChi2-7(R) | GCTTTCAAATCAACCACTCAAGACG | 25mer | ||
| PaChi1-7(F) | GAGCACAATGTCGGAGAGAAC | 21mer | Tissue expression PCR | |
| PaChi1-8(R) | ACCACTCTGCTTCAGCCACTG | 21mer | ||
| PaChi2-8(F) | GACCCCATGACTGGTGAGTGC | 21mer | ||
| PaChi2-9(R) | GTTACTCTTAGTCAGCCAGTC | 21mer |