| STR locus | Primers [ ] (mM) | Reactive | Final [ ] | PCR conditions | Temp ˚C | Time | Cycles |
Multiplex 1 | DYS391 | 0.8 | Buffer | 1X | Pre-denaturalization | 95 | 10 min | 30 |
DYS437 | 0.7 | MgCl2 | 1.75 mM | Denaturalization | 94 | 1 min | ||
DYS439 | 0.048 | dNTP | 200 mM | Annealing | 55 | 1 min | ||
DYS448 | 0.016 | Betaina | 0.83 M | Extension | 70 | 1 min | ||
DYS458 | 0.9 | TaqPol | 1 U | Post-extension | 60 | 45 min | ||
Multiplex 2 | DYS19 | 1.2 | Buffer | 1X | Pre-denaturalization | 95 | 10 min | 30 |
DYS393 | 0.2 | MgCl2 | 1.75 mM | Denaturalization | 94 | 1 min | ||
DYS438 | 0.25 | dNTP | 100 mM | Annealing | 55 | 1 min | ||
GATA-H4 | 0.4 | Betaina | 0.83 M | Extension | 70 | 1 min | ||
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| TaqPol | 1 U | Post-extension | 60 | 45 min | ||
Multiplex 3 | DYS389I | 0.25 | Buffer | 1X | Pre-denaturalization | 95 | 10 min | 30 |
DYS389II | 0.25 | MgCl2 | 1.75 mM | Denaturalization | 94 | 1 min | ||
DYS390 | 0.2 | dNTP | 100 mM | Annealing | 55 | 1 min | ||
DYS392 | 0.5 | Betaina | 0.83 M | Extension | 70 | 1 min | ||
DYS456 | 0.2 | TaqPol | 1 U | Post-extension | 60 | 45 min | ||
DYS635 | 0.5 |
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