Sperm

Storage Method¹

Incubation time, h

Characteristic²

Fresh

4

8

Cryo

SEM

P-value

Motility, %

79.6^a

32.3^b

8.1^c

6.1^c

1.86

0.0001

Progressive, %

63.3^a

26.5^b

3.8^c

3.0^c

1.64

0.0001

Rapid, %

76.0^a

29.4^b

5.1^c

3.8^c

1.75

0.0001

Static, %

10.8^b

19.3^b

38.2^a

41.5^a

3.73

0.0001

VAP, µm/s

120.0^a

80.3^b

67.4^c

75.4^b

1.88

0.0001

VSL, µm/s

99.4^a

69.6^b

56.7^c

62.2^c

1.83

0.0001

VCL, µm/s

199.3^a

123.5^b

106.1^c

122.9^c

3.06

0.0001

ALH, µm

6.8^a

5.6^b

5.4^b

7.1^a

0.15

0.0001

BCF, Hz

35.8^a

30.7^b

26.5^c

24.7^c

0.74

0.0001

STR, %

83.3^a

82.7^ab

81.1^ab

81.1^b

0.6

0.015

LIN, %

53.3

55.7

53.3

54.2

0.71

0.06

ELONG, %

43.3

43.5

41.3

44.2

0.7

0.047

AREA, µm²

5.9

6.2

5.7

6.5

0.23

0.116