Reagents | Volume (µl)×1 | Final Concentration |
Master Mix, 2× conc. | 10 µl | 1× conc. |
Mgcl2, 25 mM | 1.6 µl | 2 mM |
Primer mix, 20 × conc. {4 µM} | 1.0 µl | 0.2 µM |
Template DNA | 5.0 µl | 5 - 30 ng |
Water, PCR-grade | 2.4 µl | - |
Total volume | 20 µl | - |