| Column | Hypersil BDS C18 | Symmetry C18 | ||
| 250 × 4.6 mm, 5 µm | 100 × 2.0 mm, 2.4 µm | 150 × 4.6 mm, 5 µm | 75 × 4.6 mm, 3.5 µm | |
| Flow rate (mL) | 1.5 | 0.3 | 1.5 | 1.5 |
| Injection volume (µL) | 20 | 5 | 5 | 5 |