Response of zygotic embryos

Sucrose (M) Pretreatment

Exposure to loading/vitrification solution

Development (%)

Length (mm)

Chlorosis (%)

Necrosis (%)

0.3

LS

100.0

24.5

-

-

0.5

LS

93.0

12.3

-

7.0

0.75

LS

0.0

9.7

17.0

83.0

0.3

0.3

0.3

60% PVS2

70.0

12.6

10.0

20.0

80% PVS2

60.0

15.0

13.0

27.0

100% PVS2

47.0

15.8

23.0

30.0

0.5

0.5

0.5

60% PVS2

53.0

17.1

-

47.0

80% PVS2

10.0

12.1

37.0

53.0

100% PVS2

47.0

17.9

40.0

13.0

0.75

0.75

0.75

60% PVS2

27.0

13.6

13.0

60.0

80% PVS2

-

7.6

13.0

87.0

100% PVS2

3.0

15.6

17.0

80.0