| Response of zygotic embryos | ||||
Sucrose (M) Pretreatment | Exposure to loading/vitrification solution | Development (%) | Length (mm) | Chlorosis (%) | Necrosis (%) |
0.3 | LS | 100.0 | 24.5 | - | - |
0.5 | LS | 93.0 | 12.3 | - | 7.0 |
0.75 | LS | 0.0 | 9.7 | 17.0 | 83.0 |
0.3 0.3 0.3 | 60% PVS2 | 70.0 | 12.6 | 10.0 | 20.0 |
80% PVS2 | 60.0 | 15.0 | 13.0 | 27.0 | |
100% PVS2 | 47.0 | 15.8 | 23.0 | 30.0 | |
0.5 0.5 0.5 | 60% PVS2 | 53.0 | 17.1 | - | 47.0 |
80% PVS2 | 10.0 | 12.1 | 37.0 | 53.0 | |
100% PVS2 | 47.0 | 17.9 | 40.0 | 13.0 | |
0.75 0.75 0.75 | 60% PVS2 | 27.0 | 13.6 | 13.0 | 60.0 |
80% PVS2 | - | 7.6 | 13.0 | 87.0 | |
100% PVS2 | 3.0 | 15.6 | 17.0 | 80.0 |