P10547 D 283A

VD

1

1

DF

1

0

−1

P10547 Y 327A

WY

1

0

YM

1

0

−2

P10547 H 329A

MH

2

0

HL

2

0

−4

P10547 T 353A

ST

5

4

TG

4

4

−1

P10547 H 360A

PH

1

1

HL

2

0

−2

P10547 H 362A

LH

1

0

HF

1

0

−2

2) Original pairs after mutation

O33599 N 117A

DN

1

2

NN

4

3

0

O33599 H 210A

AH

2

2

HY

1

1

0

O33599 D 214A

VD

2

1

DY

2

1

−2

O33599 H 291A

PH

0

0

HV

0

1

1

O33599 H 293A

VH

0

1

HF

0

0

1

P10547 H 279A

MH

1

0

HY

1

0

−2

P10547 D 283A

VD

0

1

DF

0

0

1

P10547 Y 327A

WY

0

0

YM

0

0

0

P10547 H 329A

MH

1

0

HL

1

0

−2

P10547 T 353A

ST

4

4

TG

3

4

1

P10547 H 360A

PH

0

0

HL

1

0

−1

P10547 H 362A

LH

0

0

HF

0

0

0

3) Mutated pairs before mutation

O33599 N 117A

DA

4

2

AN

3

3

−2

O33599 H 210A

AA

3

3

AY

2

3

1

O33599 D 214A

VA

0

1

AY

2

3

2

O33599 H 291A

PA

1

1

AV

1

1

0

O33599 H 293A

VA

0

1

AF

0

1

2

P10547 H 279A

MA

0

1

AY

0

2

3

P10547 D 283A

VA

1

5

AF

0

1

5

P10547 Y 327A

WA

0

1

AM

0

1

2

P10547 H 329A

MA

0

1

AL

3

2

0

P10547 T 353A

SA

3

4

AG

5

4

0

P10547 H 360A

PA

0

3

AL

3

2

2

P10547 H 362A

LA

2

2

AF

0

1

1

4) Mutated pairs after mutation

O33599 N 117A

DA

5

2

AN

4

3

−4

O33599 H 210A

AA

4

4

AY

3

3

0

O33599 D 214A

VA

1

2

AY

3

3

1

O33599 H 291A

PA

2

1

AV

2

2

−1

O33599 H 293A

VA

1

2

AF

1

1

1

P10547 H 279A

MA

1

1

AY

1

2

1