Component

Concentration

Tris

50 mM

MgCl2

3 mM

Each dNTP (dATP, dCTP, dGTP, dTTP)

200 µM

Nonacetylated BSA (Sigma)

500 µg/mL

LCGreen® Plus (BioFire Diagnostics)

Melting DNA (see Table 4)

2 µM