Spot No

Protein

Identification method

Characterization method

pI Mr (kDa)

2-DE/Pred. 2-DE/Pred.

A1

Gram-negative type porin*

N-Termin.seq.

22ATVYNQDGTKL32

WGSa

(peg.1781)

3.80/3.90 60.00/40.01

Pore forming outer membrane protein precursor**

NanoLC ESI QTOF

65DLGEGLSALAYAELR79

211LEAGYSQK218

Homology search

[Pasteurella multocida]

(gi 32363216, Score: 74b)

3.50/3.90 58.00/40.01

A2

Aconitate hydratase 2* (EC 4.2.1.3)

Nano-LC ESI QTOF/

(41NPPAGEEEAILDLLTNR57

58VPPGVDEAAYVK69

79GEAESPLIDR88

324HGTDEVLTTFELK336

364ESLGLAPSDVFR375

413MTTVGSQDTTGPMTR427

491MLLPDTVGTGGDSHTR506

634WMIANGYGDAR644 835YMSEFDAMAGEIYR848

WGS (peg.1297)

5.10/4.83 90.00/99.30

Aconitate hydratase**

Nano-LC ESI QTOF/

161WGQQAFDNFR170

171VVPPGTGICHQVNLEYLGK189

283 FVEFYGDGLK 292

Homology search

[C. salexigens DSM 3043]

(gi 92114180, Score:695b)

4.90/4.96 85.00/94.60

A3

Succinate-semialdehyde dehydrogenase [NADP+]

(EC 1.2.1.16)*

gi 1500766881

Nano-LC ESI QTOF/

205GVFNVVPGR213

285NAGQTCVCTNR295

WGS (peg.1111)

5.20/4.98 60.00/54.80

Succinic semialdehyde dehydrogenase**

Nano-LC ESI QTOF/

112ETLNADLPLAVDHFR126

236IAFTGSTPVGSHILK250

251CAAENIIPSTVELGGK266

340VGAQASQEQFDK351

361EEGAEFLTGGDK372

483VALEHYQQTK492

Homology search

[C. salexigens DSM 3043]

(gi 92114412, Score:96b)

4.80/4.86 59.00/51.80

A4

Acetyl-CoA acetyltransferase (EC 2.3.1.9)*

Maldi-TOF

Homology search

[Cellulophage sp. MED134]

(gi 86132046, Score:80b)

+

WGS (peg.1731)

5.90/6.86 43.00/42.80

Acetyl-CoA C-acetyltransferase **

Maldi-TOF

Homology search

[C. salexigensDSM 3043]

(gi 92115183, Score: 79b )

5.90/5.64 42.00/40.20

A5

Malate dehydrogenase (EC 1.1.1.37)*

NanoLC ESI QTOF/

24IASGDMLGK32

234GASSAASAASAAIDHMR250

WGS

(peg.1792)

5.00/5.00 38.00/35.10

Malate dehydrogenase**

NanoLC ESI QTOF/

99KDLLEANAAIFSAQGK114

100DDLLEANAA9IFSAQGK114

212DWYENDFIPTVQQR225

Homology search

[P. bermudensis HTCC2503]

(gi 84701538, Score: 109b )

5.20/4.92 36.00/34.40

A6

Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1)*

NanoLC ESI QTOF/

91MKPSEFER98

274SLFFYVK280

WGS

(peg.1722)

4.00/4.16 50.00/37.20

Periplasmic phosphate binding protein**

NanoLC ESI QTOF/

182DAFEELVMEAASEEMDAYGGEGYTDIR208

Homology search

[C. salexigens DSM 3043]

(gi 92113600, Score: 94b )

4.00/4.16 50.00/37.20

A7

Acetyl-coenzyme A synthetase

(EC 6.2.1.1)*

NanoLC ESI QTOF/

71edgylnvshncldr84

362aimaagdelaqaadsssl380

WGS

(peg.898)

4.50/4.99 65.00/71.80

Acetate-CoA ligase**

NanoLC ESI QTOF/

201DNVDAALTR209

506DEDGYYWITGR516

Homology search

[H. chejuensis KCTC 2396]

(gi 83647747, Score: 136b)

4.50/5.40 63.00/71.80