RG-108, TSA, 8-BrAMPc, rolipram and bFGF

Human bone marrow

adult

Induction with 200 nM TSA, 3 μM RG-108, 300 μM 8-BrcAMP, 1 μM rolipram and 20 ng bFGF.

3 weeks

44% demonstrated by positive immunofluorescence for MAP2.

Neuronal morphology was observed under a microscope.

Decreased expression Oct 4, Nanog, Klf4, c Myc and increased Sox 2, nestin, A2B5, NCAM, GFAP, NeuN, MAP2, Nurr1, and TH by RT-PCR.

Immunostaining for Sox-2, nestin, A2B5, NCAM, B3T, GFAP, NeuN, and MAP2.

Release of dopamine to the medium demonstrated by ELISA.

[68]

RG-108, TSA, 8-BrAMPc, rolipram and bFGF

Human bone marrow

adult

It is preconditioning in a hypoxic environment for 48 h. Subsequent induction with 200 nM TSA, 3 μM RG-108, 300 μM 8-BrcAMP, 1 μM rolipram, and 20 ng bFGF.

3 weeks

50% and 60% were demonstrated by positive immunofluorescence for TH and Nurr1, respectively.

Neuronal morphology was observed under a microscope.

Immunostaining for TH and Nurr1 and by WB.

Increased expression of Sox 2, nestin, A2B5, NCAM, GFAP, NeuN, MAP2, Nurr1, and TH by RT-PCR.

The release of dopamine to the medium increases in the presence of ATP demonstrated by ELISA.

[69]

RG-108, TSA, 8-BrAMPc, rolipram and bFGF

Adult Sprague Dawley rat bone marrow.

Exposure to 200 nM TSA, 10 μM RG-108, 10 μM 8-BrcAMP, 1 μM rolipram and 20 ng bFGF.

7 days

73.6% indicated by morphological changes of neuronal nature.

71.6%, 73.3%, and 64.2% were demonstrated by positive immunofluorescence for Tuj1, MAp2, and ChAT, respectively.

Neuronal morphology was observed under a microscope.

Immunostaining for Tuj1, ChAT and MAP2 and by WB.

Increased expression of nestin, Tuj1, MAP2, and ChAT by RT_PCR.

No

[70]