RG-108, TSA, 8-BrAMPc, rolipram and bFGF | Human bone marrow adult | Induction with 200 nM TSA, 3 μM RG-108, 300 μM 8-BrcAMP, 1 μM rolipram and 20 ng bFGF. | 3 weeks | 44% demonstrated by positive immunofluorescence for MAP2. | Neuronal morphology was observed under a microscope. Decreased expression Oct 4, Nanog, Klf4, c Myc and increased Sox 2, nestin, A2B5, NCAM, GFAP, NeuN, MAP2, Nurr1, and TH by RT-PCR. Immunostaining for Sox-2, nestin, A2B5, NCAM, B3T, GFAP, NeuN, and MAP2. | Release of dopamine to the medium demonstrated by ELISA. | [68] |
RG-108, TSA, 8-BrAMPc, rolipram and bFGF | Human bone marrow adult | It is preconditioning in a hypoxic environment for 48 h. Subsequent induction with 200 nM TSA, 3 μM RG-108, 300 μM 8-BrcAMP, 1 μM rolipram, and 20 ng bFGF. | 3 weeks | 50% and 60% were demonstrated by positive immunofluorescence for TH and Nurr1, respectively. | Neuronal morphology was observed under a microscope. Immunostaining for TH and Nurr1 and by WB. Increased expression of Sox 2, nestin, A2B5, NCAM, GFAP, NeuN, MAP2, Nurr1, and TH by RT-PCR. | The release of dopamine to the medium increases in the presence of ATP demonstrated by ELISA. | [69] |
RG-108, TSA, 8-BrAMPc, rolipram and bFGF | Adult Sprague Dawley rat bone marrow. | Exposure to 200 nM TSA, 10 μM RG-108, 10 μM 8-BrcAMP, 1 μM rolipram and 20 ng bFGF. | 7 days | 73.6% indicated by morphological changes of neuronal nature. 71.6%, 73.3%, and 64.2% were demonstrated by positive immunofluorescence for Tuj1, MAp2, and ChAT, respectively. | Neuronal morphology was observed under a microscope. Immunostaining for Tuj1, ChAT and MAP2 and by WB. Increased expression of nestin, Tuj1, MAP2, and ChAT by RT_PCR. | No | [70] |