| BHA/ DMSO | Human umbilical cordfetal | Pre-induction in DMEM/SFB medium 20% and β-Me 3 mM for 24 h. Subsequent induction with2% DMSO and BHA200 μM. | 8 hours | 70% demonstrated by morphological changes of neuronal nature.71.4% and 73.2% were demonstrated by positive immunofluorescence for NSE and NF, respectively. | Neuronal morphology was observed under a microscope—immunostaining for NSE and NF.Presence of Nissl bodies by staining with toluidine blue. | No | [58]
|
| BHA/ DMSO | Adult human bone marrow | Pre-induction in DMEM/SFB medium 20% and β-Me 1 mM for 24 h. Subsequent induction with 2% DMSO and BHA200 μM. | 7 days. | 90%, 88%, and 90% were demonstrated by positive immunofluorescence for NSE, NF-200, and β-tubulin III, respectively. | Neuronal morphology was observed under a microscope. Immunostaining for NSE, NF-200, and B-III | No | [59] |
| TMP | Human umbilical cordFetal | Induction with 4.67 mg/ml of TMP. | 90 minutes | 85% demonstrated by morphological changes of neuronal nature.80.8% and 79.8% were demonstrated by positive immunofluorescence for NF-H and NSE, respectively. | Neuronal morphology was observed under a microscope. Novo expression of NF-H and NSE by RT-PCR. Immunostaining for NF-H and NSE and by WB. | No | [60]
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