Inductor | Origin of CMMs | Steps | Time | Percentage of differentiation reported | Technique used to demonstrate differentiation | Functionality | Reference |
Shh, FGF8, and bFGF in CMM with REST drop. | The bone marrow of adult humans. | Ip RNA vectors were transfected against REST in CMM before induction with 250 ng/ml of SHH, 100 ng/ml of FGF8, and 50 ng/ml of bFGF. | 12 days | 90% and 68.7% demonstrated by Positive Immunofluorescence for NeuN AND TH, respectively. | Neuronal morphology was observed under a microscope. Increased TH, NaV11, NeuN, Pitx3, and Nurr1 by WB. | Presence of spontaneous discharges and spontaneous postsynaptic currents demonstrated by Patch-clamp. | [99]
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