Inductor

Origin of CMMs

Steps

Time

Percentage of differentiation reported

Technique used to demonstrate differentiation

Functionality

Reference

Shh, FGF8, and bFGF in CMM with REST drop.

The bone marrow of adult humans.

Ip RNA vectors were transfected against REST in CMM before induction with 250 ng/ml of SHH, 100 ng/ml of FGF8, and 50 ng/ml of bFGF.

12 days

90% and 68.7% demonstrated by Positive Immunofluorescence for NeuN

AND TH, respectively.

Neuronal morphology was observed under a microscope.

Increased TH, NaV11, NeuN, Pitx3, and Nurr1 by WB.

Presence of spontaneous discharges and spontaneous postsynaptic currents demonstrated by Patch-clamp.

[99]