| bFGF, PDGF, Herguline, Forskolin, B-Me and RA | The bone marrow of adult Wistar rat. | Pre-induction with β-Me 1 mM and 35 ng/ ml of RA for 3 days. Subsequent induction with forskolin 5 mM, bFGF 10 ng/ml, PDGF 5 ng/ml and heregulin 200 ng/ml. | 7 days | 40% demonstrated by morphological changes of a glial nature. | Glial morphology was observed under a microscope. Immunostaining for p75, GFAP, O4, and S-100. | Post-transplant myelination in sectioned rat sciatic nerves. | [52]
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| bFGF, PDGF, Herguline, Forskolin, B-Me and RA | The umbilical cord of a fetal human. | Pre-induction with β-Me 1 mM and 35 ng/ ml of RA for 3 days. Subsequent induction with forskolin 14 μM, bFGF 10 ng/ml, PDGF 5 ng/ml and heregulin 200 ng/ml. | 8 days | 68%, 77%, 75% and 72% demonstrated by positive immunofluorescence for GFAP, p75, S100, and MBP, respectively. | Glial morphology was observed under a microscope. Immunostaining for GFAP, p75, S100, and MBP. Increased expression p75, S100B, and GFAP by RT-PCR. Increase p75 and GFAP by WB. | Improvement in the number and length of neurites when cultured with neurons from the dorsal root ganglia. | [73]
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| bFGF, FGF8, and BDNF | The bone marrow of adult Balb/c mouse. | Induction with 100 ng/ml bFGF, 10 ng/ml FGF-8 and 10 ng/ml BDNF. | 14 days | 69.9%, 30% and 50% demonstrated by positive immunofluorescence for NF200, TH and GABA, respectively. | Neuronal morphology was observed under a microscope. Immunostaining for NF200, Tau, MAP2, GFAP, Gal-C, dopadecarboxylase, TH, serotonin, and GABA. RT-PCR increased the expression of Otx2, Otx1, Pax2, Pax5, Nestin, MAP2, and Tau. | No | [74]
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| FGF-2, EGF, RA, and β-NGF | Adult Charles River mouse bone marrow | Pre-induction with FGF-2 40 ng/ ml and EGF 20 ng/ ml for 7 days. Subsequent induction with RA 0.5 μM and 20 ng/ml β-NGF | 2 weeks | 36% and 31% were demonstrated by positive immunofluorescence for Tuj1 and TUC4, respectively. | Neuronal morphology was observed under a microscope. Presence of Tuj1 and NeuN by WB. Immunostaining for B-III, TUC4, NR2A, calcium channel α1A, DCX, NeuN, MBP, GABA, Synaptophysin, Glutamic ac. Decarboxylase, Neuro D, α1B calcium channel, nestin, GFAP, and MAP2. | No | [75]
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