| Parameters | Software condition | ||
| Condition 1 | Condition 2 | Condition 3 | |
| Maximum sperm size (pixels) | 1000 | 1000 | 1000 |
| Minimum track length (frames) | 10 | 10 | 10 |
| Maximum sperm velocity between frames (pixels) | 30 | 60 | 90 |
| Minimum VCL for motility (µm/s) | 1 | 1 | 1 |
| Minimum VAP for motility (µm/s) | 1 | 1 | 1 |
| Minimum VSL for motility (µm/s) | 1 | 1 | 1 |
| Frame rate (frame per second) | 10 | 10 | 10 |