Run No.

Media concentration % (w/v)

Chitosan (g/L)

Glucose (A)

Tryptone (B)

Yeast extract (C)

Actual^a

Predicted

1

2.50

1.10

1.60

0.21

0.18

2

1.50

1.60

1.10

1

0.99

3

2.00

1.60

1.60

0.83

0.84

4

1.50

1.60

2.10

0.66

0.64

5

2.50

2.10

1.60

0.29

0.29

6

2.00

1.60

1.60

0.86

0.84

7

2.00

1.60

1.60

0.89

0.84

8

2.00

2.10

1.10

0.65

0.63

9

2.00

2.10

2.10

0.48

0.48

10

2.50

1.60

2.10

0.44

0.45

11

2.00

1.10

2.10

0.32

0.34

12

2.00

1.60

1.60

0.84

0.84

13

1.50

1.10

1.60

0.54

0.54

14

2.50

1.60

1.10

0.52

0.54

15

2.00

1.10

1.10

0.64

0.64

16

2.00

1.60

1.60

0.78

0.84

17

1.50

2.10

1.60

0.54

0.57