| Substrate | 20 | 30 | 40 | 50 | 60 | 70 | Mean |
| ˚C | |||||||
| PNP | 2.19 | 1.38 | 1.61 | 1.65 | 1.25 | 0.42 | 1.42 |
| PNP2A2E | 2.09 | 1.43 | 1.70 | 1.54 | 1.10 | 0.44 | 1.38 |
| PNPBC | 2.56 | 1.86 | 1.72 | 1.51 | 0.67 | 0.50 | 1.47 |
| Bis-PNP | 1.42 | 1.29 | 1.25 | 1.22 | 0.77 | 0.63 | 1.10 |
| DG6PNa | 2.32 | 2.78 | 1.92 | 1.41 | 1.07 | 0.20 | 1.62 |
| DG6P2Na | 2.2 | 1.05 | 3.95 | 1.43 | 0.43 | 0.57 | 1.61 |