Differences

GPMT according to Magnussona and Kligmana from 1969

Updated GPMT according

to the OECD Guideline for the Testing

of Chemicals-Skin sensitisation

Use of Dinitrobenzene

Yes

No

Propolis concentration

5%, 50%, 25%

20%, 20%, 5%

Vehicle

Propylene glycol in intradermal injection and ethanol in topical application

Ethanol in intradermal injection and topical application

Induction

Intradermal application

5% propolis solution in propylene glycol

20% propolis solution in ethanol

Topical application

50% propolis solution in ethanol

20% propolis solution in ethanol

Challenge

25% propolis solution in ethanol

5% propolis solution in ethanol

FCA

NO

YES

Observation

I observation (time not mentioned)

48 h and 72 h after challenge exposure