Differences | GPMT according to Magnussona and Kligmana from 1969 | Updated GPMT according | |
to the OECD Guideline for the Testing | |||
of Chemicals-Skin sensitisation | |||
Use of Dinitrobenzene | Yes | No | |
Propolis concentration | 5%, 50%, 25% | 20%, 20%, 5% | |
Vehicle | Propylene glycol in intradermal injection and ethanol in topical application | Ethanol in intradermal injection and topical application | |
Induction | Intradermal application | 5% propolis solution in propylene glycol | 20% propolis solution in ethanol |
Topical application | 50% propolis solution in ethanol | 20% propolis solution in ethanol | |
Challenge | 25% propolis solution in ethanol | 5% propolis solution in ethanol | |
FCA | NO | YES | |
Observation | I observation (time not mentioned) | 48 h and 72 h after challenge exposure |