| SI. No. | Components | Stock Solution (g/L of dH2O) | Quantity Used in BBM | Quantity Used in Modified BBM | Concentration in Final Medium (M) |
|
| Macronutrients |
|
|
|
|
| 1 | NaNO3 | 25.00 | 10 mL | 10 mL | 2.94 × 10−3 |
| 2 | CaCl2∙2H2O | 2.50 | 10 mL | 10 mL | 1.70 × 10−4 |
| 3 | MgSO4∙7H2O | 7.50 | 10 mL | 10 mL | 3.04 × 10−4 |
| 4 | K2HPO4 | 7.50 | 10 mL | 10 mL | 4.31 × 10−4 |
| 5 | KH2PO4 | 17.50 | 10 mL | 10 mL | 1.29 × 10−3 |
| 6 | NaCl | 2.50 | 10 mL | 10 mL | 4.28 × 10−4 |
|
| Alkaline EDTA Solution |
| 1 mL | 1 mL |
|
| 7 | EDTA | 50.00 |
|
| 1.71 × 10−4 |
| 8 | KOH | 31.00 |
|
| 5.53 × 10−4 |
|
| Acidified Iron Solution |
| 1 mL | 1 mL |
|
| 9 | FeSO4∙7H2O | 4.98 |
|
| 1.79 × 10−5 |
| 10 | H2SO4 | 1 mL |
|
|
|
|
| Boron Solution |
| 1 mL | 1 mL |
|
| 11 | H3BO3 | 11.42 |
|
| 1.85 × 10−4 |
|
| Trace Metals Solution |
| 1 mL | 1 mL |
|
| 12 | ZnSO4∙7H2O | 8.82 |
|
| 3.07 × 10−5 |
| 13 | MnCl2∙4H2O | 1.44 |
|
| 7.28 × 10−6 |
| 14 | MoO3 | 0.71 |
|
| 4.93 × 10−6 |
| 15 | CuSO4∙5H2O | 1.57 |
|
| 6.29 × 10−6 |
| 16 | Co(NO3)2∙6H2O | 0.49 |
|
| 1.68 × 10−6 |
|
| Fertilizer Solution |
|
|
|
|
| 17 | Urea | 100 | Nil | 10 mL | 1.6 × 10−2 |
| 18 | KCl | 100 | Nil | 10 mL | 1.3 × 10−2 |
| 19 | NH4PO4 | 50 | Nil | 10 mL | 4.4 × 10−3 |