Primer set

Gene

Primer name

Nucleotide sequence (5’ - 3’)

Amplicon size (bp)

Annealing temperature (˚C)

Gen bank accession #

1

26S rRNA

Forward Primer 26SF

CACAATGATAGGAGGAGCCGAC

534

60˚C

AY283368

Reverse Primer 26SR

CAAGGGAACGGGCTTGGCAGAATC

2

actin11 (for rice)

Forward Primer Act1F

CAGCCACACTGTCCCCATCTA

67

60˚C

AK100267

Reverse Primer Act1R

AGCAAGGTCGAGACGAAGGA

3

β-tubulin (for sugarcane)

Forward Primer TubF

CCAAGTTCTGGGAGGTGATCTG

103

60˚C

CA222437

Reverse Primer TubR

TTGTAGTAGACGTTGATGCGCTC