| Name | Sequences | Tm | Binding sites (nt) | Expected band |
| VNN-F1 | 5’GGATTTGGACGTGCGACCAA 3’ | 70.3 | 166 | 198 bp |
| VNN-R | 5’CTGAATTTCGAACTCCAGTG 3’ | 54.1 | 363 | |
| EcoRI F | 5¢-GATGCGAATTCGGTCGGAACCTCG-3’ | 76.4 | 442,909 | 984 bp |
| AvrII R | 5’-GCCTCAGTCACCTAGGTTAGAATAGCAGGTACGAC-3’ | 73.9 | 443,892 | |
| SnaBIF | 5’-GACTGCTACGTAATGGTACGCAAAGGTGAGAAGAA-3’ | 75.4 | 4 | 1011 bp |
| Mfel R | 5’-CACGTCACAATTGGTTTTCCGAGTCAACCCTAGTG-3’ | 79 | 1014 | |
| α-Factor F | 5’-TACTATTGCCAGCATTGCTGC-3’ | 65 | 1152 | 195 bp |
| 3’AOX1 R | 5’-GCAAATGGCATTCTGACATCC-3’ | 66.5 | 1347 |