| 10−5 M SPM | Peak (nm) | Intensity (a.u.) | Peak (nm) | Intensity (a.u.) | Peak (nm) | Intensity (a.u.) |
| 0 mL | 286.2 | 3.8 | 306.8 | 18 |
|
|
| 1 mL | 285.9 | 4 | 308.2 | 15.9 |
|
|
| 2 mL | 285.6 | 3.5 | 306.8 | 13.1 |
|
|
| 5 mL | 284.8 | 3.6 | 307 | 8.9 |
|
|
| 10 mL | 284.5 | 3.5 | 308.6 | 5.7 | 422.7 | 0.58 |
| 15 mL | 283.9 | 3.7 | 307.7 | 5.1 | 421.9 | 0.87 |
| 20 mL | 284 | 3.2 | 306.9 | 4 | 422.8 | 1.17 |
| 50 mL | 286.5 | 3.37 | 309.4 | 2.2 | 426.6 | 0.67 |