Parameter | Setting/condition |
|
Injection conditions of the injector | Volume injected | 5 μl |
Temperature | 70˚C | |
Purge rate | 60 mL/min | |
Purge time | 2.53 min | |
Total flow rate | 62.5 mL/min | |
Pressure | 30 psi | |
Gas type | Helium | |
Oven conditions | Initial temperature | 70˚C |
Initial time | 2 min | |
First gradient | 13˚C/min to 200˚C, isothermal for 0 min | |
Second gradient | 5˚C/min to 290˚C, isothermal for 5 min | |
Conditions of the column | HP 5MS Agilent 19091S-433UI | 30 m × 0.25 mm, 0.25 μm |
Carrier gas flow rate | 1 mL/min, constant flow | |
carrier gas | Helium (99.9995% high purity grade) | |
Conditions of the transfert line | Temperature | 325˚C |
Mass spectrometer conditions | Temperature of the SM source | 230˚C |
Temperature of the SM quadrupole | 180˚C | |
Solvent delay | 5 min | |
Ionization energy | 70 eV |