Parameter

Setting/condition

Injection conditions of the injector

Volume injected

5 μl

Temperature

70˚C

Purge rate

60 mL/min

Purge time

2.53 min

Total flow rate

62.5 mL/min

Pressure

30 psi

Gas type

Helium

Oven conditions

Initial temperature

70˚C

Initial time

2 min

First gradient

13˚C/min to 200˚C, isothermal for 0 min

Second gradient

5˚C/min to 290˚C, isothermal for 5 min

Conditions of the column

HP 5MS Agilent 19091S-433UI

30 m × 0.25 mm, 0.25 μm

Carrier gas flow rate

1 mL/min, constant flow

carrier gas

Helium (99.9995% high purity grade)

Conditions of the transfert line

Temperature

325˚C

Mass spectrometer conditions

Temperature of the SM source

230˚C

Temperature of the SM quadrupole

180˚C

Solvent delay

5 min

Ionization energy

70 eV