Std

Run

Factor 1

Factor 2

Factor 3

Response 1

Response 2

Response 3

Response 4

Response 5

A:Flow

B: Temperature

C:Mobile phase A pH

Rs Imp C & Imp B

Rs DABE & Imp H

RS DABE TF

AY DABE TF

AY DABE TP

7

1

0.8

35

4.7

4.72

6.22

1.09

1.02

56,339

6

2

1.2

25

4.7

3.43

6.19

1.09

1.06

42,754

8

3

1.2

35

4.7

4.67

6.53

1.07

1.02

49,162

1

4

0.8

25

4.3

1.74

5.45

1.14

1.15

45,773

5

5

0.8

25

4.7

3.49

5.63

1.12

1.06

49,076

3

6

0.8

35

4.3

2.7

6.01

1.13

1.11

52,866

9

7

1

30

4.5

2.97

6.07

1.12

1.08

46,174

11

8

1

30

4.5

2.98

6.14

1.11

1.08

46,515

10

9

1

30

4.5

3.06

6.17

1.1

1.08

46,662

2

10

1.2

25

4.3

1.74

5.94

1.11

1.14

40,369

4

11

1.2

35

4.3

2.52

6.45

1.08

1.11

46,234

12

12

0.8

30

4.5

3.09

5.84

1.11

1.08

50,272

13

13

1.2

30

4.5

3.05

6.43

1.11

1.08

43,373

14

14

1

25

4.5

2.48

5.96

1.12

1.1

43,178

15

15

1

35

4.5

3.66

6.39

1.1

1.06

50,296

16

16

1

30

4.3

2.19

6.13

1.14

1.12

46,166

17

17

1

30

4.7

4.13

6.17

1.09

1.04

49,514