| Critical method attributes | Critical method parameters | |||||
| Flow | Column temperature | Mobile phase-A buffer concentration | Mobile phase-A pH | Mobile phase organic ratio | Injection volume | |
| Resolution between Impurity C and B | High | High | Low | High | Low | Low |
| Resolution between Impurity DABE and H | Medium | Medium | Low | Medium | Low | Low |
| DABE RS Standard Tailing Factor | Medium | Medium | Low | Medium | Low | Low |
| DABE AY Standard Tailing Factor | Medium | Medium | Low | Medium | Low | Low |
| DABE AY Theoretical Plates | Medium | Medium | Low | Medium | Low | Low |