| Urine validation results | Methamphetamine | Amphetamine | Cathinone | Methcathinone | ||||
| S(+) | R(−) | S(+) | R(−) | R(+) | S(−) | R(+) | S(−) | |
| Extraction recovery | 94% | 98% | 86% | 85% | 70% | 70% | 85% | 86% |
| Matrix effect | 114% | 106% | 108% | 109% | 111% | 113% | 118% | 117% |
| Precision RSD (intra-day) (6) | 2.7% | 3.7% | 3.4% | 2.3% | 4.0% | 4.4% | 3.8% | 3.2% |
| Precision RSD (inter-day) (6 + 6 + 6) | 2.8% | 4.0% | 2.8% | 3.2% | 3.6% | 3.7% | 4.3% | 3.3% |
| Lower limit of detection (pg/mL) | 20 | 20 | 200 | 200 | 250 | 250 | 20 | 20 |
| Lower limit of confirmation (pg/mL) | 50 | 50 | 500 | 500 | 500 | 500 | 50 | 50 |
| Plasma validation results | Methamphetamine | Amphetamine | Cathinone | Methcathinone | ||||
| S(+) | R(−) | S(+) | R(−) | R(+) | S(−) | R(+) | S(−) | |
| Extraction recovery | 100% | 97% | 95% | 98% | 84% | 84% | 100% | 94% |
| Matrix effect | 109% | 112% | 118% | 117% | 98% | 112% | 113% | 123% |
| Precision RSD (intra-day) (6) | 3.7% | 2.2% | 4.3% | 4.7% | 3.5% | 2.9% | 3.8% | 3.7% |
| Precision RSD (inter-day) (6 + 6 + 6) | 4.5% | 4.6% | 4.8% | 4.6% | 3.9% | 2.9% | 4.3% | 3.2% |
| Lower limit of detection (pg/mL) | 2 | 2 | 20 | 20 | 10 | 10 | 2 | 2 |
| Lower limit of confirmation (pg/mL) | 5 | 5 | 50 | 50 | 25 | 25 | 5 | 5 |