Parameter

Condition opted

Concentration

RF

Mean (%) ± SD

% RSD

Volume of mobile phase

12 mL

800 ng/spot

0.53

892.78 ± 2.06

1.014

15 mL

0.52

881.89 ± 1.09

0.69

18 mL

0.55

902.93 ± 1.57

0.838

Saturation time of development chamber

20 min

800 ng/spot

0.54

876.89 ± 2.35

0.952

30 min

0.50

905.21 ± 1.88

1.090

40 min

0.56

886.67 ± 1.45

0.615