Parameter | Condition opted | Concentration | RF | Mean (%) ± SD | % RSD |
Volume of mobile phase | 12 mL | 800 ng/spot | 0.53 | 892.78 ± 2.06 | 1.014 |
15 mL | 0.52 | 881.89 ± 1.09 | 0.69 | ||
18 mL | 0.55 | 902.93 ± 1.57 | 0.838 | ||
Saturation time of development chamber | 20 min | 800 ng/spot | 0.54 | 876.89 ± 2.35 | 0.952 |
30 min | 0.50 | 905.21 ± 1.88 | 1.090 | ||
40 min | 0.56 | 886.67 ± 1.45 | 0.615 |