| S. No. | Mobile Phase Used | Observation | |
| Solvents | Ratios |
| |
| 1 | Butanol:Ethanol:Water | 8:7:4 | No spots |
| 2 | Butanol:Glacial acetic acid:Water | 9.5:0.2:0.3 | Tailing |
| 3 | Chloroform:Ethanol:Water | 8:1:1 | No spots |
| 4 | Methanol:Acetonitrile | 7:3 | Spots obtained but not resolved properly |
| 5 | Methanol:Chloroform | 7:3 | No tailing |
| 6 | Methanol:Chloroform | 6:4 | Well defined spots |