| S. No. |
Mobile Phase Used |
Observation |
|
| Solvents |
Ratios |
|
|
| 1 |
Butanol:Ethanol:Water |
8:7:4 |
No spots |
| 2 |
Butanol:Glacial acetic acid:Water |
9.5:0.2:0.3 |
Tailing |
| 3 |
Chloroform:Ethanol:Water |
8:1:1 |
No spots |
| 4 |
Methanol:Acetonitrile |
7:3 |
Spots obtained but not resolved properly |
| 5 |
Methanol:Chloroform |
7:3 |
No tailing |
| 6 |
Methanol:Chloroform |
6:4 |
Well defined spots |