Matrix

Spiked amount (ng/mL)

Stability (%)

Freeze-thaw stability

Short-term stability

Long-term stability

Post-preparative stability

Plasma

(?20˚C, 3 cycles)

(ice-cold condition, 2 hours)

(?20˚C, 9 weeks)

(4˚C, 24 hours)

16

102.5

99.6

90.8

110.7

800

100.6

101.1

88.8

100.5

Lung

(?20˚C, 2 cycles)

(ice-cold condition, 2 hours)

(?20˚C, 4 weeks)

(4˚C, 24 hours)

homogenate

30

90.0

94.0

86.6

98.5

800

89.7

94.1

92.9

101.6

(?20˚C, 3 cycles)

(?20˚C, 6 weeks)

30

82.7

80.5

800

81.4

90.6