Matrix

Time (min)

Compound

Precursor ion (m/z)

Product ion (m/z)

Scan time (sec)

Collision energy (eV)

Plasma

0 - 4.1

Delamanid

535

352

0.5

30

4.1 - 6.0

IS

454

208

0.5

30

Spray voltage 4.5 kV; electron multiplier voltage 1.5 kV; nitrogen sheath gas pressure 70 psi; nitrogen auxiliary gas pressure 10 (arbitrary units); argon collision gas pressure 2.0 m Torr; capillary temperature 240˚C.

Lung

homogenate

0 - 7.5

(cycle scan)

Delamanid

535

352

0.3

33

IS

454

208

0.3

27

Spray voltage 4.5 kV; electron multiplier voltage 2.5 kV; nitrogen nebulizer gas 14 (arbitrary units); nitrogen curtain gas 6 (arbitrary units); nitrogen collision gas 12 (arbitrary units); heated gas pressure 70 psi; heated gasflow rate 7 L/min; heated gas temperature 475˚C.