| Number | Substance | Retention time (min) |
| 1 | Acetic acid | 4.09 |
| 2 | Propionic acid | 5.00 |
| 3 | Isobutyric acid | 5.32 |
| 4 | Butyric acid | 6.04 |
| 5 | Isovaleric acid | 6.54 |
| 6 | Valeric acid | 7.39 |
| 7 | Crotonic acid | 7.89 |
| 8 | Caproic acid | 8.81 |